Device
Part:BBa_K1092025
Designed by: Group: iGEM13_Hong_Kong_CUHK (2013-09-18)
T7-RBS -ssDsbA-PDZ Ligand-Voltage Switch-Laccase
This biobrick is the counterpart of the part: T7-RBS-ssDsbA-PDZ Domain-Voltage sensor peptide-Dioxygenase (BBa_K1092026), and is composed of the voltage switch (BBa_K1092009 & BBa_K1092010) and the Dioxygenase (BBa_K1092002) and Laccase (BBa_K1092004). In this example, we explore the possiblity of using our novel voltage switch as a fast control of reaction rate. We made use of the Laccase and Dioxygenase as an example to illustrate this.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 929
Illegal AgeI site found at 2447 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 550
Illegal BsaI.rc site found at 2608
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Categories
Parameters
None |